【文獻標題】Physiological-biochemical responses of wheat to blending controlled-release potassium chloride ａnd soluble potassium chloride【作者】Zeli Li , Weitao Zhang , Lixue Qiu ,et.al
【作者單位】山東農(nóng)業(yè)大學（ Shandong Agricultural University）
【文獻中引用產(chǎn)品】
植物生長素（IAA）ELISA試劑盒
植物脫落酸(ABA)ELISA試劑盒
【關(guān)鍵詞】Root scanner ，Root characteristics ，Photosynthetic rate ，Endogenous hormone ，Potassium
【DOI】https://doi.org/10.1016/j.still.2021.105058
【影響因子(IF)】8.6
【出版期刊】《Soil & Tillage Research》
【產(chǎn)品原文引用】
2.3. Sampling ａnd chemical analyses
The yields were five consecutive years (2014–2019), so we explained the mechanism of increasing yield of controlled-release potassium chloride through physiological ａnd biochemical indexes during growing seasons 2018? 2019. Samples from undisturbed surface soil (0–20 cm) were collected at seedling stage (31 days after fertilizing), re-greening stage (157 days after fertilizing), ａnd after dressing at jointing stage (188 days after fertilizing), grain-filling stage (210 days after fertilizing) ａnd at maturity (245 days after fertilizing). At jointing ａnd grain-filling stages, relative chlorophyll content (SPAD value) was determined by a chlorophyll meter (SPAD-502, Minolta, Japan) ａnd conducted by Li et al. (2019). The photosynthesis of the ear leaf was measured by a Li-6400XT portable photosynthesis system (Licor Inc., Lincoln, USA). The leaf area index (LAI) by a canopy analyzer (LAI-2200, Beijing, China) was evaluated at five different points. Leaf ａnd root samples were collected from the wheat during the jointing ａnd grain-filling growth stages. Auxin (IAA) and abscisic acid (ABA) were estimated using the ELISA kit provided by Shanghai Hengyuan Biological 114 Technology Co. Ltd. (Shanghai, China). Soil samples of plowed horizons (0–20 cm) were collected from each plot, then air-dried in the laboratory, ａnd any visible roots, organic residues, ａnd stone fragments were removed manually, used by 2-mm, ａnd 0.25-mm sieves, ａnd further analyses. The available K of soil was extracted with 1.0 mol L? 1 of CH3COONH4 (7.0 pH) ａnd analyzed with a flame photometer (MODEL410, Sherwood, England).
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